After 3 hr, the tissue suspension was filtered through a 100-μm tissue strainer and the filtrate was centrifuged for 5 min at 300 × Flow cytometry analysis was performed to determine the expression of surface markers on the cultured WBS_MSCs. Previous studies with transforming growth factor β1 (TGF-β1) demonstrated positive effects on elastin synthesis in human fibroblasts,Involvement of reactive oxygen species in TGF-beta1-induced tropoelastin expression by human dermal fibroblasts.Transforming growth factor-beta stabilizes elastin mRNA by a pathway requiring active Smads, protein kinase C-delta, and p38.Transforming growth factor-beta up-regulates elastin gene expression in human skin fibroblasts. Chie Kojima. Furthermore, the transfection of EAHy.926 cells with modified TE mRNA demonstrated no significant influence on cell viability. Afterward, Cy3 was conjugated to 5-azido-CTo isolate MSCs from a patient with WBS (WBS_MSCs), we rinsed the thymus tissue of a WBS patient with Dulbecco’s PBS (DPBS; Life Technologies, Darmstadt, Germany) and cut it into small pieces. Using Fastin assay, we detected a 1.2-fold increased amount of elastin in the skin treated with synthetic modified TE mRNA compared with the skin without mRNA treatment (2.5 μg synthetic modified TE mRNA was complexed with Lipofectamine 2000 and injected using hollow microneedles into the pig skin. Evidence for post-transcriptional modulation.Induced elastin regeneration by chronically activated smooth muscle cells for targeted aneurysm repair.Skin elastic fibres: regulation of human elastin promoter activity in transgenic mice.Transforming growth factor-beta up-regulates human elastin promoter activity in transgenic mice.Elastin stabilizes an infarct and preserves ventricular function.Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats.Treatment of burn and surgical wounds with recombinant human tropoelastin produces new elastin fibers in scars.Transforming growth factor beta stimulates mammary adenocarcinoma cell invasion and metastatic potential.Pre-existing immunity against vaccine vectors—friend or foe?.Viral vectors: a look back and ahead on gene transfer technology.Interaction between the elastin peptide VGVAPG and human elastin binding protein.Nature and the multiple functions of the 67-kD elastin-/laminin binding protein.Vascular wall extracellular matrix proteins and vascular diseases.Inhibitory effect of type 1 collagen gel containing α-elastin on proliferation and migration of vascular smooth muscle and endothelial cells.Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA.HPLC purification of in vitro transcribed long RNA.Sequence-engineered mRNA without chemical nucleoside modifications enables an effective protein therapy in large animals.Facilitated noninvasive visualization of collagen and elastin in blood vessels.In vitro synthesis of modified mRNA for induction of protein expression in human cells.Received: An α-elastin standard from 1.25 to 80 μg was used to detect the elastin quantity in the samples.To detect the produced elastin by dot blot analysis, we blotted 500 μL supernatant using the Bio-Dot SF device (Bio-Rad, Hercules, CA, USA) on nitrocellulose membrane according to the manufacturer’s instructions. Padma Pradeepa Srinivasan, Vaishali N. Patel, Shuang Liu, Daniel A. Harrington, Matthew P. Hoffman, Xinqiao Jia, Robert L. Witt, Mary C. Farach-Carson, Swati Pradhan-Bhatt. Journal of Biomedical Materials Research Part B: Applied BiomaterialsO. Differences of p < 0.05 were considered significant.M.L., R.M.P., and M.A.-A. Collagen Membrane and Immune Response in Guided Bone Regeneration: Recent Progress and Perspectives. Primary Salivary Human Stem/Progenitor Cells Undergo Microenvironment-Driven Acinar-Like Differentiation in Hyaluronate Hydrogel Culture. Sufficient amount of elastin in the human body is important for maintaining proper functioning of the cardiovascular system, blood vessels, internal organs which are systematically subjected to compression and tension such as a heart, lungs, bladder, skin, tendons, and sphincters.Total amount of elastin and collagen in human body reaches 35% from total amount of proteins, which are required to ensure the following things:Elastin is produced by human body, but its production decreases with time. Data are presented as box plot, median with 25–75 percentiles (box) and 5–95 percentiles (whiskers) (n = 7). Afterward, round pieces with a diameter of 15 mm were punched out. By continuing you agree to the Because this protein is physiologically synthesized only to a limited extent after the neonatal period, elastin deficiencies caused by degenerative changes or genetic defects lead to significant functional limitations in the affected organs or tissues. The latter results in reduction of elastin’s and collagen’s synthesis by 30%. Here, we applied synthetic modified mRNA encoding tropoelastin (TE) for the de novo synthesis of elastin and determined the mRNA-mediated elastin synthesis in cells, as well as ex vivo in porcine skin. Recombinant Resilin-Based Bioelastomers for Regenerative Medicine Applications. Regulation of Epithelial-to-Mesenchymal Transition Using Biomimetic Fibrous Scaffolds. The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting One-way ANOVA followed by Bonferroni’s multiple comparison test was performed to compare the means of more than two groups. In situ self-assembly of mild chemical reduction graphene for three-dimensional architectures.
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